Detection of fusion genes resulting from chromosome abnormalities in childhood acute lymphoblastic leukemia / 中华医学遗传学杂志
Chinese Journal of Medical Genetics
; (6): 551-553, 2005.
Article
in Zh
| WPRIM
| ID: wpr-280004
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To detect the expression of the fusion genes resulting from chromosome abnormalities in childhood acute lymphoblastic leukemia(ALL) and its conformity to WHO classification.</p><p><b>METHODS</b>Sixty-two children with ALL were investigated. The expression of fusion genes was determined by multiplex reverse transcription-polymerase chain reaction (RT-PCR), karyotyping (R band) and immunophenotyping (by flow cytometry) were also performed.</p><p><b>RESULTS</b>Of the 62 patients, 23(37.1%) were found to carry 13 different fusion genes. The patients with immunophenotype of Pre-B-ALL were found to carry: TEL/AML1(3 cases); E2A/PBX1, E2A/HLF, TLS/ERG, MLL/AF4, MLL/AF9, MLL/AF10, MLL/AFX-MLL/AF6-MLL/ELL, MLL/AF6-MLL/ELL, dupMLL (one case for each); and HOX11 (6 cases). The patients with immunophenotype of Pre-T-ALL were found to carry: TAL1D (4 cases, one is also found to have HOX11 expression); and HOX11 (2 cases). The multiplex RT-PCR in combination with chromosome analysis revealed genetic abnormalities in 69.4%(43/62) of childhood ALL.</p><p><b>CONCLUSION</b>Multiplex RT-PCR combined with chromosome analysis and immunophenotyping can provide reliable and helpful information for the diagnosis, therapy evaluation and prognosis prediction in childhood ALL, which may also serve as a basis on which to implement the criteria of WHO classification.</p>
Full text:
1
Index:
WPRIM
Main subject:
Transcription Factors
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Oncogene Proteins, Fusion
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Immunophenotyping
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Chromosome Aberrations
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Proto-Oncogene Proteins
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Homeodomain Proteins
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Reverse Transcriptase Polymerase Chain Reaction
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RNA-Binding Protein FUS
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DNA-Binding Proteins
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Myeloid-Lymphoid Leukemia Protein
Type of study:
Diagnostic_studies
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Prognostic_studies
Limits:
Adolescent
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Child
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Child, preschool
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Humans
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Infant
Language:
Zh
Journal:
Chinese Journal of Medical Genetics
Year:
2005
Type:
Article