Sex chromosome analysis and IGF-II expression on activated human unfertilized oocytes after ICSI with calcium ionophore A23187 and puromycin / 中华医学遗传学杂志
Chinese Journal of Medical Genetics
;
(6): 524-527, 2005.
Article
in Chinese
| WPRIM
| ID: wpr-280010
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the sex chromosomes and the expression of insulin-like growth factor-II (IGF-II) on activated human unfertilized oocytes after intracytoplasmic sperm injection(ICSI) with calcium ionophore A23187 and puromycin.</p><p><b>METHODS</b>All 95 discarded oocyes that showed no evidence of fertilization at 16-18 h after in vitro maturation and intracytoplasmic sperm injection cycles (IVM-ICSI)/conventional ICSI were exposed to calcium ionophore A23187 (5 micromol/L) for 5 min and then were incubated with puromycin (10 microg/mL) for 4 h. After activation, the oocytes were cultured in vitro for 3-5 days. The sex chromosome analysis was performed by dual color fluorescence in situ hybridization. The expression of IGF-II on the activated embryos, normal embryos, and parthenotes was examined.</p><p><b>RESULTS</b>The combination of calcium ionophore A23187 with puromycin could activate the unfertilized oocytes 22 h after ICSI. The activated rate, cleavage rate, and quality of activated embryos of the IVM-ICSI group were similar to those of ICSI group, respectively. Sex chromosome analysis indicated that 8 male and 5 female embryos had been derived from two pronucleus and a second polar body. The expression of IGF-II on activated embryos and normal embryos was high and similar, which was much stronger than that of parthenotes.</p><p><b>CONCLUSION</b>The combination of calcium ionophore A23187 with puromycin could effectively activate unfertilized oocytes 22 h after ICSI. Moreover, the unfertilized oocytes activated by calcium ionophore A23187 and puromycin had normal sex chromosomes and expression of IGF-II like the normal embryos. These suggest that oocyte activation may be considered as a remedial measure in the presence of total or nearly total fertilization failure in ICSI.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Oocytes
/
Pharmacology
/
Insulin-Like Growth Factor II
/
Puromycin
/
Calcimycin
/
In Situ Hybridization, Fluorescence
/
Sperm Injections, Intracytoplasmic
/
Cell Biology
/
Chromosomes, Human, X
/
Chromosomes, Human, Y
Limits:
Female
/
Humans
Language:
Chinese
Journal:
Chinese Journal of Medical Genetics
Year:
2005
Type:
Article
Similar
MEDLINE
...
LILACS
LIS