Identification and characterization of a novel gene EOLA1 stimulating ECV304 cell proliferation / 中华医学遗传学杂志
Chinese Journal of Medical Genetics
; (6): 518-523, 2005.
Article
in Zh
| WPRIM
| ID: wpr-280011
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To amplify the full-length cDNA and characterize the structure and biological function of a novel expression sequence tag ST55 (GenBank Accession No. BM121646).</p><p><b>METHODS</b>Rapid amplification of cDNA ends was used to clone the full-length of cDNA of ST55 in this study. Then, its tissue distribution was checked by Northern blots, and the associated protein was screened by GAL 4-based yeast two-hybrid. The effect of stable transfection of the cDNA on cell proliferation was evaluated in ECV304 cells.</p><p><b>RESULTS</b>A full-length 1404 bp cDNA was cloned, and it was accepted as a novel human mRNA by GenBank (No. AY074889), named endothelial-overexpressed lipopolysaccharide-associated factor 1 (EOLA1). Bioinformatic analysis found that the EOLA1 encoded 158 amino acids, 17.89 kDa protein, and mapped to chromosome Xq27.4 with 5 exons. EOLA1 expressed in different human normal tissues and cancer cell lines. Using the EOLA1 cDNA as bait, we performed a yeast two-hybrid screening of a human liver cDNA library and identified metallothionein 2A (MT2A) as associated protein. The interaction between EOLA1 and MT2A was confirmed by co-immunoprecipitation experiments. Stable transfection of EOLA1 was noted to stimulate ECV304 cell proliferation (P < 0.05).</p><p><b>CONCLUSION</b>The findings suggest that EOLA1 is a novel gene and the interaction of EOLA1 and MT2A may play an important role in cell protection in inflammation reaction.</p>
Full text:
1
Index:
WPRIM
Main subject:
Physiology
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Protein Binding
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Molecular Sequence Data
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Base Sequence
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Cell Line
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Blotting, Western
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Blotting, Northern
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Exons
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Sequence Alignment
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Amino Acid Sequence
Type of study:
Prognostic_studies
Limits:
Humans
Language:
Zh
Journal:
Chinese Journal of Medical Genetics
Year:
2005
Type:
Article