Development of a SPA-ELISA method for detecting anti-coronavirus IgG antibodies in serum samples from fulvous fruit bats / 南方医科大学学报
Journal of Southern Medical University
;
(12): 736-738, 2008.
Article
in Chinese
| WPRIM
| ID: wpr-280107
ABSTRACT
<p><b>OBJECTIVE</b>To develop an simple and sensitive method for detecting anti-coronavirus IgG antibodies in bat sera based on enzyme-linked immunosorbent assay (ELISA).</p><p><b>METHODS</b>A commercial ELISA kit for detecting SARS-CoV antibody was modified for detecting coronavirus antibodies in bat serum samples. The second antibody in the kit was replaced with horseradish peroxidase-conjugated protein-A (HRP-SPA) based on the characteristics of binding between Staphylococcus aureus protein A (SPA) and mammal IgG Fc fragment. The sera of 55 fulvous fruit bats (Rousettus dasymallus) were tested using the SPA-ELISA.</p><p><b>RESULTS</b>The test results of the positive and negative controls in the kit and the serum samples from convalescent ;patient were consistent with expectation. Coronavirus antibody was detected in 2 out of the 55 bat serum samples. Serum neutralization test confirmed the validity of the SPA-ELISA method.</p><p><b>CONCLUSION</b>This SPA-ELISA method is applicable for detecting coronavirus antibody in bat sera.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Virology
/
Blood
/
Immunoglobulin G
/
Enzyme-Linked Immunosorbent Assay
/
Chiroptera
/
Coronavirus
/
Allergy and Immunology
/
Methods
/
Antibodies, Viral
Limits:
Animals
Language:
Chinese
Journal:
Journal of Southern Medical University
Year:
2008
Type:
Article
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