Function of F10 gene, a novel hydatidiform mole-related gene: a preliminary study / 南方医科大学学报
Journal of Southern Medical University
;
(12): 722-724, 2008.
Article
in Chinese
| WPRIM
| ID: wpr-280111
ABSTRACT
<p><b>OBJECTIVE</b>To study the function of F10 gene, a novel hydaditiform mole-related gene.</p><p><b>METHODS</b>A549 cell line was transfected with the F10 gene of forward or reverse sequence or with the empty vector, respectively. The cellular mRNA was extracted after 24 h of transfection to screen for the differentially expressed genes among the 3 transfected and the control cells using differential display-polymerase chain reaction (ddPCR).</p><p><b>RESULTS</b>The bands representing differentially expressed genes were amplified from the cells, and the products were linked to T-Vector for sequence analysis. Several genes were screened by Blasting and their expressions were confirmed by fluorescent quantitative PCR.</p><p><b>CONCLUSION</b>F10 gene is functionally related to cell proliferation and apoptosis.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Oncogenes
/
Pathology
/
Uterine Neoplasms
/
Transfection
/
Hydatidiform Mole
/
Gene Expression Regulation, Neoplastic
/
Apoptosis
/
Hydatidiform Mole, Invasive
/
Gene Expression Profiling
/
Cell Line, Tumor
Limits:
Female
/
Humans
/
Pregnancy
Language:
Chinese
Journal:
Journal of Southern Medical University
Year:
2008
Type:
Article
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