Effect of N-acetylcysteine on HMGB1 and RAGE expression in the lungs of asthmatic mice / 南方医科大学学报
Journal of Southern Medical University
;
(12): 692-695, 2008.
Article
in Chinese
| WPRIM
| ID: wpr-280119
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the expression of HMGB1 and RAGE mRNA in the lungs of asthmatic mice and the effect of N-acetylcysteine (NAC) on their expression.</p><p><b>METHODS</b>Twenty-one female BALB/c mice were randomly divided into control group, asthma group and NAC group (n=7). The expressions of HMGB1 and RAGE mRNA and their distributions in the lungs were detected by RT-PCR and immunohistochemical method.</p><p><b>RESULTS</b>The expression levels of HMGB1 and RAGE mRNA were not significantly different between the control group (0.88-/+0.02 and 1.20-/+0.20, respectively) and the asthma model group (0.86-/+0.05 and 1.21-/+0.08, P>0.05). After NAC treatment, both of HMGB1 and RAGE mRNA levels (0.98-/+0.05 and 1.58-/+0.21) were significantly higher than those in the other two groups (P<0.05). HMGB1 was found in the nuclei and membrane of the bronchial and alveolar epithelial cells, and RAGE was located on the membrane of the alveolar epithelial cells.</p><p><b>CONCLUSION</b>HMGB1 and RAGE may play a role in the oxidative stress during asthma, but the exact mechanism needs further investigation.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Pharmacology
/
Acetylcysteine
/
Asthma
/
RNA, Messenger
/
Immunohistochemistry
/
Random Allocation
/
Gene Expression
/
Free Radical Scavengers
/
Reverse Transcriptase Polymerase Chain Reaction
Limits:
Animals
Language:
Chinese
Journal:
Journal of Southern Medical University
Year:
2008
Type:
Article
Similar
MEDLINE
...
LILACS
LIS