Effect of Tongxinluo on the proliferation and differentiation of rat embryonic neural stem cells / 南方医科大学学报
Journal of Southern Medical University
;
(12): 679-683, 2008.
Article
in Chinese
| WPRIM
| ID: wpr-280122
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of Tongxinluo in on the proliferation and differentiation of rat embryonic neural stem cells (NSCs).</p><p><b>METHODS</b>NSCs were isolated from 12- to 14-day SD rat embryo and treated with Tongxinluo at different doses, and the proliferation and differentiation of the cells were observed by immunofluorescence staining at different time points.</p><p><b>RESULTS</b>The ratio of embryonic NSCs labeled with nestin decreased soon after Tongxinluo treatment, but increased afterwards. Significant difference was noted in the number of cells labeled with beta-tubulin between Tongxinluo group and the control group 3 and 7 days after the treatment, and also between high-dose and low-dose Tongxinluo groups at 7 days.</p><p><b>CONCLUSION</b>Tongxinluo can induce the proliferation and neuronal differentiation of rat embryonic NSCs, and the effect is related to the dose of Tongxinluo administered.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Drugs, Chinese Herbal
/
Cell Differentiation
/
Cells, Cultured
/
Fluorescent Antibody Technique
/
Rats, Sprague-Dawley
/
Cell Biology
/
Cell Proliferation
/
Dose-Response Relationship, Drug
/
Embryonic Stem Cells
Limits:
Animals
/
Pregnancy
Language:
Chinese
Journal:
Journal of Southern Medical University
Year:
2008
Type:
Article
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