Hydrocamptothecin promotes the mRNA expression of Wnt signaling inhibitor DKK-1 / 南方医科大学学报
Journal of Southern Medical University
;
(12): 623-626, 2008.
Article
in Chinese
| WPRIM
| ID: wpr-280133
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effects of hydrocamptothecin on the expression of Wnt signaling pathway inhibitor DKK-1 in tumor cells.</p><p><b>METHODS</b>Human HepG2, Hep3B, LoVo and U251 cells were treated with the antitumor drug Hydrocamptothecin. DKK-1 mRNA expression in the cells was detected with RT-PCR, and beta-catenin expression was measured by fluorescence-activated cell sorting (FACS).</p><p><b>RESULTS</b>DKK mRNA in Hep3B, HepG2, LoVo and U251 cells was significantly increased after hydrocamptothecin treatment for 24 h, and the percentage of beta-catenin-positive cells and fluorescence intensity for beta-catenin expression was lowered in the cells after the treatment.</p><p><b>CONCLUSION</b>Hydrocamptothecin promotes mRNA expression of Wnt signaling pathway inhibitor DKK-1 in Hep3B, HepG2, LoVo and U251 cells.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Pharmacology
/
Camptothecin
/
RNA, Messenger
/
Colorectal Neoplasms
/
Signal Transduction
/
Reverse Transcriptase Polymerase Chain Reaction
/
Intercellular Signaling Peptides and Proteins
/
Cell Line, Tumor
/
Wnt Proteins
Limits:
Humans
Language:
Chinese
Journal:
Journal of Southern Medical University
Year:
2008
Type:
Article
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