Effect of Tween-80 on the permeability of rhodamine 123, a P-gp substrate across rat intestinal membranes in vitro / 南方医科大学学报
Journal of Southern Medical University
;
(12): 579-581, 2008.
Article
in Chinese
| WPRIM
| ID: wpr-280144
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of Tween-80 in modulating rhodamine123 (R123) permeability across the intestinal membranes.</p><p><b>METHODS</b>The permeability of R123 or fluorescein sodium (CF) across isolated rat intestinal membranes at the concentration of 5 microg/ml was evaluated using an in vitro diffusion chamber system, in the presence or absence of Tween-80 at different low concentrations. The concentration of R123 or CF in the receptor chamber was determined using fluorospectrophotometry.</p><p><b>RESULTS</b>The intestinal membrane permeability of R123 gradually decreased from the jejunum to the ileum and then to the colon. The serosal-to-mucosal transport of R123 was much greater than its mucosal-to-serosal transport. In the presence of low concentrations of Tween-80, the absorptive transport of R123 was significantly increased while its secretory transport decreased depending on the concentration of Tween-80. However, Tween-80 at the experimental concentrations was found to obviously affect the CF transport across the intestinal membranes.</p><p><b>CONCLUSION</b>Low concentrations of Tween-80 may promote the absorption of P-gp-mediated drugs and therefore improve the oral bioavailability of these drugs.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Permeability
/
Pharmacology
/
Polysorbates
/
In Vitro Techniques
/
Pharmacokinetics
/
Rats, Wistar
/
Colon
/
ATP Binding Cassette Transporter, Subfamily B, Member 1
/
Rhodamine 123
/
Ileum
Limits:
Animals
Language:
Chinese
Journal:
Journal of Southern Medical University
Year:
2008
Type:
Article
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