Influence of DNAzymes against cyclin D1 in tumor cell cycle / 生物医学工程学杂志
Journal of Biomedical Engineering
;
(6): 374-384, 2009.
Article
in Chinese
| WPRIM
| ID: wpr-280196
ABSTRACT
In this study, DNAzymes against cyclin D1 (cyclin D1-DRz) were designed according to the secondary structure of cyclin D1 mRNA which was computed with RNAdraw and Mfold. Cyclin D1-DRz were transfected into tumor cell line u251 and HeLa by oligofectamine. The expression of cyclin D1 was detected by RT-PCR. It was shown that the expression of cyclin D1 gene was suppressed obviously, and the expressions of other cell-cycle related genes such as cyclin E1, cyclin A1 and cyclin B1 were also declined. The cell cycle analysis of tumor cells tansfected with cyclin D1-DRz revealed an arrestment in the G0/G1 phase. In conclusion, the approach is effective and feasible for designing DNAzyme. Cyclin D1-DRz is useful for interfering with the cell cycle procession of tumor cells.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
RNA, Messenger
/
Molecular Sequence Data
/
Base Sequence
/
HeLa Cells
/
Transfection
/
Cell Cycle
/
Cyclin D1
/
DNA, Catalytic
/
Genetics
Limits:
Humans
Language:
Chinese
Journal:
Journal of Biomedical Engineering
Year:
2009
Type:
Article
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