Differentiation of bone marrow mesenchymal stem cells co-cultured with endothelial cells under shear stress / 生物医学工程学杂志
Journal of Biomedical Engineering
;
(6): 85-88, 2009.
Article
in Chinese
| WPRIM
| ID: wpr-280258
ABSTRACT
Differentiation of bone marrow mesenchymal stem cells (BMSCs) co-cultured with endothelial cells (ECs) under shear stress was studied. BMSCs and ECs were co-cultured on the two sides of PET membrane, and 20 dyn/cm2 shear stress produced by parallel plate flow chamber was performed after 72 hours. Cell morphology was observed under phase-difference microscope, and the expressions of smooth muscle-alpha-actin (SM-alpha-actin), calponin and smooth muscle myosin heavy chain (SMMHC) of BMSCs were detected by fluorescence immunocytochemistry. The co-cultured BMSCs became smooth muscle-like cells gradually; after 24 hours, the BMSCs started to express SM-alpha-actin. After 48 hours, they expressed SM-alpha-actin and calponin obviously. After 72 hours, obvious expressions of SM-alpha-actin and calponin, but not of SMMHC, were detected. Further static co-culture had no effect on SM-alpha-actin, calponin and SMMH expression of BMSCs; after 24 hours, shear stress induced feeble expression of SM-alpha-actin and obvious expression of SMMHC in co-cultured BMSCs, but it had no effect on the expression of calponin. The results suggest that shear stress may potentiate the differentiation of BMSCs (co-cultured with ECs) into mature smooth muscle-like cells.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Stress, Mechanical
/
Calcium-Binding Proteins
/
Bone Marrow Cells
/
Cell Differentiation
/
Cells, Cultured
/
Actins
/
Rats, Sprague-Dawley
/
Myosin Heavy Chains
/
Coculture Techniques
/
Cell Biology
Limits:
Animals
Language:
Chinese
Journal:
Journal of Biomedical Engineering
Year:
2009
Type:
Article
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