Establishment of a One-Step Real-Time RT-PCR Method for the Detection of Venezuelan Equine Encephalitis Virus / 病毒学报
Chinese Journal of Virology
;
(6): 107-113, 2015.
Article
in Chinese
| WPRIM
| ID: wpr-280287
ABSTRACT
Venezuelan equine encephalitis (VEE) is a zoonotic disease caused by the Venezuelan equine encephalitis virus (VEEV) complex. This disease has not yet been reported in China, and it is therefore essential to establish a rapid and accurate method for detection of the virus in order to prevent and control this disease. In this study, a one-step real-time quantitative RT-PCR method was developed for the detection of the VEEV complex. A pair of specific primers and a Taqman probe were designed corresponding to a conserved region of the VEEV gene nspl, allowing the detection of all known strains of different sub- types of the virus. Using RNA synthesized by in vitro transcription as template, the sensitivity of this method was measured at 3.27 x 10(2) copies/microL. No signal was generated in response to RNA from Chikungunya virus (CHIKV), nor to RNA encoding the nsp1 fragment of Eastern equine encephalitis virus (EE-EV) or Western equine encephalitis virus (WEEV), all of which belong to the same genus as VEEV. This indicates that the method has excellent specificity. These results show that this one-step real-time quantitative RT-PCR method may provide an effective tool for the detection of VEEV in China.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Virology
/
RNA, Viral
/
China
/
Classification
/
DNA Primers
/
Reverse Transcriptase Polymerase Chain Reaction
/
Encephalitis Virus, Venezuelan Equine
/
Encephalomyelitis, Venezuelan Equine
/
Genetics
/
Methods
Type of study:
Diagnostic study
Limits:
Humans
Country/Region as subject:
South America
/
Asia
/
Venezuela
Language:
Chinese
Journal:
Chinese Journal of Virology
Year:
2015
Type:
Article
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