The suppressive effect of MiR-490 on coxsackievirus B3 replication / 病毒学报
Chinese Journal of Virology
; (6): 619-623, 2014.
Article
in Zh
| WPRIM
| ID: wpr-280318
Responsible library:
WPRO
ABSTRACT
To study the effect of miR-490 on Coxsackievirus B3 (CVB3) replication, HeLa cells were trans- fected with miR-490 in vitro, and infected with a Renilla luciferase (RLuc)-expressing CVB3 variant (RLuc-CVB3). The activities of RLuc in these cells were measured at 8h intervals from 0 to 40 h post-infection (p.i.), and the effects of miR-490 on RLuc-CVB3 replication were observed. In a further study, HeLa cells were transfected with either miR-490 or antisense miR-490 (AMO-miR-490), and were then infected with an enhanced green fluorescence protein (EGFP)-expressing CVB3 variant (EGFP-CVB3). The replication of EGFP-CVB3 was then determined by detecting the expression of EGFP. We observed that miR-490 could significantly inhibit the expression of RLuc in infected cells at 32 h p. i. Furthermore, in HeLa cells infected with EGFP-CVB3 at 32 h p.i., EGFP expression was also significantly inhibited by the presence of mniR-490. The inhibitory effect of miR-490 on EGFP expression in EGFP-CVB3-infected cells could be reversed by tranfection with AMQ-miR-490. These results indicated that miR-490 significantly inhibits the replication and expression of QVB3.
Full text:
1
Index:
WPRIM
Main subject:
Physiology
/
Virology
/
Virus Replication
/
HeLa Cells
/
Enterovirus B, Human
/
MicroRNAs
/
Enterovirus Infections
/
Genetics
/
Metabolism
Limits:
Humans
Language:
Zh
Journal:
Chinese Journal of Virology
Year:
2014
Type:
Article