Research methods in protein-protein and protein-nucleic acid interactions and application in the study of human enterovirus A71 / 病毒学报
Chinese Journal of Virology
;
(6): 587-593, 2014.
Article
in Chinese
| WPRIM
| ID: wpr-280323
ABSTRACT
Methods for analyses of protein-protein interactions include yeast two hybrid (Y2H), phage dis- play (PD), co-immunoprecipitation (Co-IP), glutathione S-transferase pull-down (GST pull-down), cellular co-localization, far-western blotting, virus overlay protein binding assay (VOPBA), surface plasmon resonance (SPR), and fluorescence resonance energy transfer (FRET). Technologies for the detection of protein-nucleic acid interactions include yeast one hybrid (Y1H), chromatin immunoprecipitation (ChIP), electrophoretic mobility shift assay (EMSA), Southwestern blotting, reporter gene, Co-IP, GST pull-down, and PD. These methods are often used in the study of the human enterovirus A71 (EV-A71) by our research team. Reviews in the Chinese literature in this field are lacking, so we reviewed applications of these methods in the study of EV-A71. This review may impart important knowledge in the research of other viruses with regard to protein-protein and protein-nucleic acid interactions.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Viral Proteins
/
RNA, Viral
/
Chemistry
/
Two-Hybrid System Techniques
/
Enterovirus A, Human
/
Electrophoretic Mobility Shift Assay
/
Fluorescence Resonance Energy Transfer
/
Genetics
/
Metabolism
Language:
Chinese
Journal:
Chinese Journal of Virology
Year:
2014
Type:
Article
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