Characterization of the whole genome from a human parechovirus type 3 detected from the serum of a child with sepsis in Beijing, China / 病毒学报
Chinese Journal of Virology
;
(6): 541-548, 2014.
Article
in Chinese
| WPRIM
| ID: wpr-280330
ABSTRACT
Human parechovirus type 3 (HPeV3) is an important pathogen of severe sepsis. HPeV3 is a non- enveloped, single-stranded, positive-sense RNA virus with a linear and continuous genomic RNA. The complete genome of a HPeV3 (BJ-C3174) strain was analyzed from the serum specimen from a child with sepsis hospitalized in Beijing, China, in 2012. The whole genome of BJ-C3174 was 7329 nucleotides (nt) in length excluding a poly (A) tail. One large open reading frame (ORF) of 6531 nt encoding a putative polyprotein precursor of 2177 amino acids (aa) was flanked by a 5' untranslated region (UTR) of 709 nt and 3' UTR of 91 nt. Phylogenetic analysis showed that BJ-C3174 belonged to HPeV3 and was closest to the HPeV3 strain BONN-2 from Germany. Compared with HPeV1-8 reference strains, BJ-C3174 shared the highest similarities with BONN-2 in full length and in each of the gene segments of the genome. The nucleotide and predicted amino acid identities of the whole genome between BJ-C3174 and BONN-2 were 99.3% and 99.8%, respectively, which were higher than those compared with HPeV3 prototype. Recom- bination of the gene segment with other HPeVs types was not identified.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Phylogeny
/
Virology
/
Blood
/
Molecular Sequence Data
/
Amino Acid Sequence
/
Classification
/
Genome, Viral
/
Sepsis
/
Parechovirus
/
Genetics
Limits:
Child
/
Humans
Language:
Chinese
Journal:
Chinese Journal of Virology
Year:
2014
Type:
Article
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