Construction of pEGFP-C1/U6-mediated plasmid expressing MDR1 shRNA / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 384-387, 2006.
Article
in Chinese
| WPRIM
| ID: wpr-280660
ABSTRACT
To construct a plasmid expressing MDR1 short hairpin RNA (shRNA) mediated by pEGFP-C1/U6 vector, two coding sequences of 19 nucleotides were selected from MDR. Two pairs of oligonucleotides were designed for these two fragments. After annealing the formed double-stranded DNAs were ligated with plasmid pEGFP-C1/U6 (pEGFP-C1 vector with U6 promoter). The plasmids producing MDR1 shRNA were constructed from the inverted motif containing 9 spacers and four Ts. The results showed that the constructed plasmids were named pEGFP-C1/U6/A and pEGFP-C1/U6/B, and the constructs were identified by restriction and sequence analysis, no any base mutation was observed. It is concluded that plasmids of pEGFP-C1/U6/A and pEGFP-C1/U6/B expressing MDR1 shRNA were successfully constructed, providing a highly effective method for reversing the multidrug resistance in clinic.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Plasmids
/
RNA, Messenger
/
RNA, Small Nuclear
/
ATP Binding Cassette Transporter, Subfamily B, Member 1
/
Drug Resistance, Multiple
/
Drug Resistance, Neoplasm
/
RNA, Small Interfering
/
Green Fluorescent Proteins
/
DNA-Binding Proteins
/
Genetic Vectors
Limits:
Humans
Language:
Chinese
Journal:
Journal of Experimental Hematology
Year:
2006
Type:
Article
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