Establishment of delta block matching technique / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 366-368, 2006.
Article
in Chinese
| WPRIM
| ID: wpr-280664
ABSTRACT
To establish delta block HLA-matching technique, DNA was extracted from whole blood by salting-out method, delta block was amplified by polymerase chain reaction (PCR), and PCR product was detected by GeneScan. The results showed that delta block had polymorphism in 104 samples without sibship of the Han people from Zhejiang province. The range of DNA fragment length was 81-393 bp and could be divided into 4 groups 81-118 bp, 140-175 bp, 217-301 bp, 340-393 bp. The numbers of DNA fragments were 6-32. It is concluded that the method of delta block matching is reliable and can be applied to select donors for the patients to be transplanted. It is the first time to get delta block data of the Han people in China.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Histocompatibility Testing
/
HLA-A Antigens
/
HLA-B Antigens
/
HLA-DQ Antigens
/
HLA-DR Antigens
/
Hematopoietic Stem Cell Transplantation
/
Allergy and Immunology
/
HLA-DRB1 Chains
/
Genetics
/
Methods
Limits:
Humans
Language:
Chinese
Journal:
Journal of Experimental Hematology
Year:
2006
Type:
Article
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