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Detection of expression of Th1/Th2 cytokines in jurkat cell treated with gamma-interferon by cytometric bead array / 中国实验血液学杂志
Article in Chinese | WPRIM | ID: wpr-280667
Responsible library: WPRO
ABSTRACT
In order to explore the value of gamma-interferon (IFN-gamma) in therapy of T lymphoid leukemia and role of cytometric bead array (CBA) method in detection of Th1/Th2 cytokines expression, the Jurkat cells were cultured for 48 hours in different concentration of IFN-gamma, the expressions of IL-2, IL-6, INF-gamma and TNF-alpha in culture supernatants were assayed by CBA method; CD25 expression was assayed by flow cytometry. The results showed that the expressions of IL-2, TNF-alpha were enhanced in INF-gamma dose-dependent way, and the expression of CD25 was also enhanced, but there was no expression of IL-6. It is concluded that Jurkat cells induced by IFN-gamma were able to express high-level of Th1 cytokine and IL-2 membrane receptor (CD25), and the CBA method can be used to exactly evaluate the dynamic change of Th1/Th2 cytokines.
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Pharmacology / Humans / Enzyme-Linked Immunosorbent Assay / Sensitivity and Specificity / Interferon-gamma / Interleukin-2 / Tumor Necrosis Factor-alpha / Th2 Cells / Th1 Cells / Jurkat Cells Language: Chinese Journal: Journal of Experimental Hematology Year: 2006 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Pharmacology / Humans / Enzyme-Linked Immunosorbent Assay / Sensitivity and Specificity / Interferon-gamma / Interleukin-2 / Tumor Necrosis Factor-alpha / Th2 Cells / Th1 Cells / Jurkat Cells Language: Chinese Journal: Journal of Experimental Hematology Year: 2006 Type: Article