Detection of expression of Th1/Th2 cytokines in jurkat cell treated with gamma-interferon by cytometric bead array / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 356-358, 2006.
Article
in Chinese
| WPRIM
| ID: wpr-280667
ABSTRACT
In order to explore the value of gamma-interferon (IFN-gamma) in therapy of T lymphoid leukemia and role of cytometric bead array (CBA) method in detection of Th1/Th2 cytokines expression, the Jurkat cells were cultured for 48 hours in different concentration of IFN-gamma, the expressions of IL-2, IL-6, INF-gamma and TNF-alpha in culture supernatants were assayed by CBA method; CD25 expression was assayed by flow cytometry. The results showed that the expressions of IL-2, TNF-alpha were enhanced in INF-gamma dose-dependent way, and the expression of CD25 was also enhanced, but there was no expression of IL-6. It is concluded that Jurkat cells induced by IFN-gamma were able to express high-level of Th1 cytokine and IL-2 membrane receptor (CD25), and the CBA method can be used to exactly evaluate the dynamic change of Th1/Th2 cytokines.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Enzyme-Linked Immunosorbent Assay
/
Sensitivity and Specificity
/
Interferon-gamma
/
Interleukin-2
/
Tumor Necrosis Factor-alpha
/
Th2 Cells
/
Th1 Cells
/
Jurkat Cells
/
Flow Cytometry
Type of study:
Diagnostic study
Limits:
Humans
Language:
Chinese
Journal:
Journal of Experimental Hematology
Year:
2006
Type:
Article
Similar
MEDLINE
...
LILACS
LIS