Influence of human mesenchymal stem cells on cell proliferation and chemo-sensitivity of K562 cells / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 308-312, 2006.
Article
in Zh
| WPRIM
| ID: wpr-280677
Responsible library:
WPRO
ABSTRACT
This study was aimed to compare K562 cell proliferation, chemo-sensitivity and alteration of MDR1 before and after adhesive culture with MSC, so as to evaluate the relationship between chemodrug-resistance of leukemia cells and hemopoietic microenvironment. K562 cell cultivated in suspension and adhesively cultivated with MSC were collected respectively and cell proliferation curves were drawn; the cell cycle was determined by flow cytometry; the effect of chemotherapy on cellular viability and apoptosis of K562 cell was investigated, the MDR1 gene expression was determined by RT-PCR. The results showed that K562 cells adhesively cultivated with MSC were inhibited and cells in G0/G1 increased (P < 0.05), cells in S phase decreased (P < 0.05) and those in G0/G1 increased (P < 0.01), compared with that cultivated in suspension. In process of daunomycin-inducing apoptosis, K562 cell apoptosis in the adhesive culture with MSC was inhibited (P < 0.05). MDR1 gene expression in K562 cells was not induced or altered by adhesive co-cultivation. It is concluded that by co-culture of cell-cell contact with MSC, growth suppression and induction of chemo-resistance of K562 cells take place. The mechanism, however, seems not relevant with MDR1.
Full text:
1
Index:
WPRIM
Main subject:
Pharmacology
/
Physiology
/
Bone Marrow Cells
/
Daunorubicin
/
Cells, Cultured
/
Apoptosis
/
ATP Binding Cassette Transporter, Subfamily B, Member 1
/
Drug Resistance, Multiple
/
Drug Resistance, Neoplasm
/
Coculture Techniques
Limits:
Humans
Language:
Zh
Journal:
Journal of Experimental Hematology
Year:
2006
Type:
Article