Detection of complex karyotype in a myelodysplastic syndrome cell line (MUTZ-1) by metaphase fluorescence in situ hybridization / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 46-49, 2006.
Article
in English
| WPRIM
| ID: wpr-280736
ABSTRACT
This study was aimed to investigate the cytogenetic changes of MDS cell line (MUTZ-1) with chromosome 5q deletion. R-banding analysis was used to identify chromosome aberrations in MDS cell line and Vysis Spectra Vysion M-FISH was used to further characterize chromosomal complex karyotype. The results indicated that M-FISH exhibited obvious chromosomal aberrations with high frequency including translocation, insertion, breakage and rearrangement, deletion and increasement of chromosome number, the complex karyotype of MUTZ-1 was shown as 50, xx, der (1) t (1;2), ins (1;14), +der (2) t(2;19), der (3) t (3;5), der (3) (3522), 5q-, der (6) t (3;6), der (7) (18717), +8, +der (9) t (1;9), der (10) t (1;10), +11, +12, der (?13) (101358), der (14) t (8;14), der (14) t (14, 15), der (15) t (15;21) x 2, +17, +18, -21, -22. It is concluded that M-FISH analysis revealed obvious changes in complex karyotype of MDS cell line MUTZ-1, and the M-FISH technique can increase accuracy of detection for chromosomal complex karyotype, and help diagnosis and prognostic evaluation of MDS.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Translocation, Genetic
/
Myelodysplastic Syndromes
/
Chromosomes, Human, Pair 5
/
Tumor Cells, Cultured
/
Chromosome Aberrations
/
Chromosome Deletion
/
In Situ Hybridization, Fluorescence
/
Genetics
/
Karyotyping
/
Methods
Type of study:
Diagnostic study
Limits:
Child, preschool
/
Female
/
Humans
Language:
English
Journal:
Journal of Experimental Hematology
Year:
2006
Type:
Article
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