Transplantation of mesenchymal derived stem cells followed by G-CSF injection can reconstitute hematopoiesis of lethally irradiated BALB/c mice / 中国医学科学院学报
Acta Academiae Medicinae Sinicae
;
(6): 20-24, 2002.
Article
in Chinese
| WPRIM
| ID: wpr-280972
ABSTRACT
<p><b>OBJECTIVE</b>To explore the hematopoietic reconstitution potential of mesenchymal derived stem like cells.</p><p><b>METHODS</b>We transplanted bone marrow mesenchymal derived stem like cells into lethally irradiated BALB/c mice. Hematopoietic cells were derived from the non-adherent bone marrow cells 24 hours after initial culture while murine mesenchymal derived stem like cells from bone marrow of donor mice were cultured for 10 days before the transplantation.</p><p><b>RESULTS</b>All mice of group 1 and 3 died in 7-8 days post irradiation following transplantation, while all the mice from group 2 and 4 survived. The time course of hematopoietic reconstitution was then observed. The peripheral blood and bone marrow cell count recovered in the MSC + G-CSF transplanted group and the BM transplanted group after 3 weeks. Interestingly, CFU-GM number in the MSC + G-CSF transplanted group increased significantly after 2 weeks and even more than that in the BM transplanted group after 3 weeks while as CFU-GM colony dropped 2 weeks after in the BM transplanted group. Spleen colony (CFU-S) number and size of the MSC + G-CSF transplanted group was significantly greater than the BM transplanted group. Furthermore, PCR analysis was performed using peripheral blood cells to determine if any male-derived cells were present. No male-derived cells were found in any of the mice from group 1 and 3. Y-chromosome-specific src gene was found to be dominant in the MSC + G-CSF transplanted group and the BM transplanted group by week 4 post transplantation. In addition, we demonstrated that induction with G-CSF lead to CFU-GM colony formation from MSC compartment in vitro.</p><p><b>CONCLUSION</b>These results indicate that under stimulation of G-CSF, mesenchymal derived stem like cells might differentiate into hematopoietic primitive stem cells in vivo and have the capacity to re-establish hematopoiesis in lethally irradiated mice. This study should provide an alternative transplantation treatment for malignancy.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Radiation Effects
/
Radiation Injuries, Experimental
/
Radiation-Protective Agents
/
Hematopoietic Stem Cells
/
Cells, Cultured
/
Granulocyte-Macrophage Colony-Stimulating Factor
/
Whole-Body Irradiation
/
Bone Marrow Transplantation
/
Cell Biology
/
Stem Cell Transplantation
Limits:
Animals
Language:
Chinese
Journal:
Acta Academiae Medicinae Sinicae
Year:
2002
Type:
Article
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