The effects of cytokines mediated ex vivo expansion on the cell adhesion molecule expression of cord blood hematopoietic stem and progenitor cells / 中国医学科学院学报
Acta Academiae Medicinae Sinicae
;
(6): 7-10, 2002.
Article
in Chinese
| WPRIM
| ID: wpr-280975
ABSTRACT
<p><b>OBJECTIVE</b>To compare the expression of cell adhesion molecules (CAMs) among VLA-4 (CD49 d), VLA-5 (CD49e), LFA-1 (CD11a), L-selectin (CD62L), and PECAM-1 (CD31) which are more related to the homing of hematopoietic stem and progenitor cells (HSPC) on the ex vivo expanded CD34+ subset with that of fresh isolated AC133+ cells.</p><p><b>METHODS</b>AC133+ cells selected from fresh cord blood (CB) samples were cultured in QBSF-60 serum-free media in the presence of Flt-3 ligand + SCF + TPO (FST), with initial addition of IL-3 for up to 2 week. Expansion potential and the expression of above CAMs were evaluated at day 0, day 7, day 10 and day 14.</p><p><b>RESULTS</b>(1) Simultaneously numerical expansion of various HSPC was constantly observed during the culture, and the fold expansion of AC133+ cells and CD34+ cells on day 14 were 33.50 and 64.56 respectively; (2) The number of CD34+ subsets expressing the above adhesions were all increased at different degrees (from 20 fold to 160 fold). (3) The expressions of CD11a, CD49d, and CD49e on ex vivo expanded CD34+ cells were increased as compared to their baseline levels, but the percentage of CD62L+ and CD31+ subpopulations in CD34+ cells were decreased.</p><p><b>CONCLUSIONS</b>Our short-term culture system can not merely support the simultaneous expansion of CB derived AC133+ cells, but the expanded hematopoietic progenitors may well sustained the expression of homing-related adhesion molecules.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Peptides
/
Pharmacology
/
Physiology
/
Hematopoietic Stem Cells
/
Glycoproteins
/
Antigens, CD
/
Cell Adhesion Molecules
/
Cell Division
/
Cells, Cultured
/
Cytokines
Limits:
Female
/
Humans
/
Pregnancy
Language:
Chinese
Journal:
Acta Academiae Medicinae Sinicae
Year:
2002
Type:
Article
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