Localization and differentiation of hair follicle stem cells / 中国医学科学院学报
Acta Academiae Medicinae Sinicae
;
(6): 360-363, 2006.
Article
in Chinese
| WPRIM
| ID: wpr-281198
ABSTRACT
<p><b>OBJECTIVE</b>To identify the localization of hair follicles stem cell (HFSC) in different stages of hair and explore the differentiating capacity of HFSC into epidermis in vitro.</p><p><b>METHODS</b>HFSC were detected by K19 immunostaining in normal human skin. Then, the isolated HFSC through enzyme digestion were seeded on dermal equivalent (DE) and cultured between the air-liquid interfaces for 14 days. Skin-equivalents was harvested and used for evaluation.</p><p><b>RESULTS</b>HFSC mainly located in outer root sheet in hair follicle and human anagen hair follicles containing two distinct reservoirs for K19-positive cells located in the bulge and bulb of the follicle. These two reservoirs fused in line of outer root sheets during the catagen-telogen transition phase and individualized again in the newly forming anagen hair follicle. Based on DE, growing HFSC built a multilayered and confined epidermis.</p><p><b>CONCLUSION</b>HFSC located in outer root sheets can promote hair cycle and differentiate into epidermis in vitro.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Physiology
/
Stem Cells
/
Cell Differentiation
/
Cells, Cultured
/
Hair Follicle
/
Cell Biology
/
Epidermis
Limits:
Humans
Language:
Chinese
Journal:
Acta Academiae Medicinae Sinicae
Year:
2006
Type:
Article
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