Up-regulation of Let-7a Expression Induces Gastric Carcinoma Cell Apoptosis In Vitro / 中国医学科学杂志(英文版)
Chinese Medical Sciences Journal
;
(4): 44-47, 2017.
Article
in English
| WPRIM
| ID: wpr-281420
ABSTRACT
Objective To investigate effect of let-7a gene over-expression on apoptosis of gastric cancer cell lines SGC-7901.Methods The stable let-7a gene over-expressing SGC-7901 cells, SGC-7901/let-7a cells, were established using shRNA lentiviral vector methods. Real-time RT-PCR analysis was used to evaluate the expression level of let-7a mRNA. Cells apoptosis was assessed by flow cytometry.Results RT-PCR analysis revealed let-7a expression in SGC-7901/let-7a cells was significantly increased. Cellular apoptosis assay showed that over-expression of let-7a could increase apoptosis of SGC-7901 cells (P=0.002).Conclusion Up-regulating let-7a expression promoted apoptosis in SGC-7901 cells.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Stomach Neoplasms
/
RNA, Neoplasm
/
Gene Expression Regulation, Neoplastic
/
Up-Regulation
/
Apoptosis
/
MicroRNAs
/
Cell Line, Tumor
/
Genetics
/
Metabolism
Limits:
Humans
Language:
English
Journal:
Chinese Medical Sciences Journal
Year:
2017
Type:
Article
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