X-ray irradiation enhances tumor necrosis factor receptor p55 expression in human colorectal cancer cells and inhibits the release of its soluble form in vitro / 南方医科大学学报
Journal of Southern Medical University
;
(12): 1921-1923, 2007.
Article
in Chinese
| WPRIM
| ID: wpr-281503
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effects of X ray on human colorectal cancer cells for their tumor necrosis factor receptor-p55 (TNFR-p55) expression and release of soluble soluble TNFR-p55 (sTNFR-p55) in vitro.</p><p><b>METHODS</b>The protein expression of TNFR-p55 in Lovo cells exposed to X-ray was detected using immunohistochemistry, and enzyme-linked immunosorbent assay was used to examine the levels of sTNFR-p55 in the supernatants of the cell culture. The cell apoptosis of the exposed cells was analyzed with flow cytometry, and the changes in cell morphology were observed microscopically.</p><p><b>RESULTS</b>X-ray exposure of cells resulted in a strong increase in TNFR-p55 expression of (P<0.01) and LoVo cell apoptosis (P<0.05). The levels of sTNFR-p55 in the supernatant of cells with X-ray exposure was significantly lowered in comparison with the levels before exposure (P<0.01). Optical microscopy showed that the exposed LoVo cells shrank and became spherical with cytoplasmic condensation and nuclear pyknosis.</p><p><b>CONCLUSION</b>X-ray exposure can induce LoVo cell apoptosis by increasing TNFR-p55 expression on the cell membrane and inhibiting the release of sTNFR-p55 in the supernatants.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
X-Rays
/
Enzyme-Linked Immunosorbent Assay
/
Immunohistochemistry
/
Colorectal Neoplasms
/
Gene Expression Regulation, Neoplastic
/
Apoptosis
/
Cell Line, Tumor
/
Receptors, Tumor Necrosis Factor, Type I
/
Tumor Necrosis Factor Decoy Receptors
/
Flow Cytometry
Limits:
Humans
Language:
Chinese
Journal:
Journal of Southern Medical University
Year:
2007
Type:
Article
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