Immunodetection of ginsenoside Rb1 in rat serum / 南方医科大学学报
Journal of Southern Medical University
; (12): 1915-1917, 2007.
Article
in Zh
| WPRIM
| ID: wpr-281505
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To establish a novel immunoassay for qualitative detection of ginsenoside Rb1 in rat serum.</p><p><b>METHODS</b>Anti-G-Rb1 monoclonal antibody (mAb) was through a hybridoma approach. Rat serum containing G-Rb1 was deproteinized with methanol to prepare the sample for testing, which was loaded onto polyethersulfone (PES) membrane and developed in the mixture of acetonitrile, water and acetic acid (25:75:1). After treatment with NaIO(4), the membrane was transferred to 1% BSA solution for immobilization of G-Rb1. The membrane was subsequently treated with anti-G-Rb1 mAb solution, followed by addition of peroxidase-labeled goat anti-mouse IgG and color development using 4-chloro-1-naphthol-0.03% H(2)O(2).</p><p><b>RESULTS</b>On the PES membrane, a clear blue spot representing G-Rb1 occurred where the rat serum for testing and the standard G-Rb1 samples were blotted. The limit of this immunodetection was 0.25 microg.</p><p><b>CONCLUSION</b>This immunoassay has greater specificity and reliability than thin-layer chromatography with a sensitivity similar to that of high-performance liquid chromatography, and does not require sophisticated equipment for convenient G-Rb1 detection in rat serum.</p>
Full text:
1
Index:
WPRIM
Main subject:
Blood
/
Immunoassay
/
Ginsenosides
/
Limit of Detection
/
Hybridomas
/
Antibodies, Monoclonal
Type of study:
Qualitative_research
Limits:
Animals
Language:
Zh
Journal:
Journal of Southern Medical University
Year:
2007
Type:
Article