Expression of proliferating cell nuclear antigen in severely damaged small intestinal mucosa due to high-dose 5-FU exposure / 南方医科大学学报
Journal of Southern Medical University
;
(12): 1860-1862, 2007.
Article
in Chinese
| WPRIM
| ID: wpr-281521
ABSTRACT
<p><b>OBJECTIVE</b>To detect the expression of proliferating cell nuclear antigen (PCNA) in severely damaged intestinal mucosa due to high-dose 5-FU exposure.</p><p><b>METHODS</b>Thirty-two adult C57BL/6J mice were subjected to daily intraperitoneal high-dose 5-FU injection at 150 mg/kg for 5 consecutive days, and on days 1, 3, and 5, the mice were sacrificed to obtain the small intestinal tissue for HE straining and immunohistochemistry for detecting PCNA expression. Another 8 mice with intraperitoneal PBS injection served as the control group.</p><p><b>RESULTS</b>High-dose 5-FU exposure of the mice resulted in severe intestinal mucous damage, with complete destruction of the villi and crypts and significantly increased cells positive for PCNA expression (P<0.01).</p><p><b>CONCLUSION</b>High-dose 5-FU treatment can significantly increase the PCNA index, and the cells expressing PCNA can be closely associated with regeneration of the severely damaged mucosa due to the exposure.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Proliferating Cell Nuclear Antigen
/
Fluorouracil
/
Intestinal Mucosa
/
Intestine, Small
/
Metabolism
/
Mice, Inbred C57BL
/
Antimetabolites, Antineoplastic
Limits:
Animals
Language:
Chinese
Journal:
Journal of Southern Medical University
Year:
2007
Type:
Article
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