Effect of hypoxic preconditioning on the learning and memory ability and expressions of survivin and HSP-70 proteins in rats with focal cerebral ischemia/reperfusion injury / 南方医科大学学报
Journal of Southern Medical University
;
(12): 1856-1859, 2007.
Article
in Chinese
| WPRIM
| ID: wpr-281522
ABSTRACT
<p><b>OBJECTIVE</b>To study the neuroprotective effect of hypoxic preconditioning on reperfusion injury following ischemia and its molecular mechanism.</p><p><b>METHODS</b>Forty-eight rats were randomized into 3 groups, namely the sham operated group, ischemia/reperfusion (I/R) group, and I/R following hypoxic preconditioning group (HP+I/R). In the latter two groups, the rats were subjected to middle cerebral artery occlusion (MACO) for 3 h followed by reperfusion for 24 h to induce cerebral I/R injury. The learning and memory ability of the rats 24 h after reperfusion was assessed using Y-maze test. Immunohistochemistry was performed to quantify the expressions of survivin and HSP-70 proteins in the rat brain tissues.</p><p><b>RESULTS</b>The number of survivin- and HSP-70-positive cells in the brain tissues was significantly different between HP+I/R group and IR and the sham operated groups (P<0.05), and following I/R injury, the rats in HP+I/R group showed much better performance in the Y-maze test than those in I/R group.</p><p><b>CONCLUSION</b>Hypoxic preconditioning can protect the ischemic brain against reperfusion injury, promote recovery of the learning and memory ability and neurological functions following the injury. Up-regulation of the expressions of survivin and HSP-70 proteins might be one of the molecular mechanisms for this neuroprotective effect.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Therapeutics
/
Brain
/
Reperfusion Injury
/
Brain Ischemia
/
Rats, Sprague-Dawley
/
HSP70 Heat-Shock Proteins
/
Ischemic Preconditioning
/
Infarction, Middle Cerebral Artery
/
Memory
/
Metabolism
Limits:
Animals
Language:
Chinese
Journal:
Journal of Southern Medical University
Year:
2007
Type:
Article
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