Protective effect of ketamine against acute rat lung injury induced by liopolysaccharide and its mechanism / 南方医科大学学报
Journal of Southern Medical University
;
(12): 1848-1850, 2007.
Article
in Chinese
| WPRIM
| ID: wpr-281524
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the protective effects of different concentrations of ketamine against acute lung injury induced by lipopolysaccharide (LPS) in rats and its mechanism.</p><p><b>METHODS</b>Forty-eight male Wistar rats were randomized into 4 equal groups, namely the control group, LPS group, ketamine group I (5 mg/kg), and ketamine group II (10 mg/kg). The neutrophil count, protein contents in the bronchoalveolar lavage fluid (BALF) and the wet/dry lung weight ratio were measured 4 h after LPS injection. TNF-alpha, IL-8, NO, iNOS and NF-kappaB were also measured in the lung tissues.</p><p><b>RESULTS</b>In LPS group, the neutrophil count, protein contents in BALF, the wet/dry lung weight ratio and the levels of tumor necrosis factor-alpha(TNF-alpha), interleukin-8 (IL-8), and NO were all significantly increased compared with the control group (P<0.01). The mRNA expression of iNOS and the protein expression of NF-kappaB were also increased in LPS groups. Ketamine treatment attenuated the increase in wet/dry lung weight ratio, neutrophil count, and protein contents in BALF in a dose-dependent manner. Ketamine also dose-dependently inhibited the production of TNF-alpha, IL-8 , and NO and lowered iNOS mRNA and NF-kappaB protein expression.</p><p><b>CONCLUSION</b>Ketamine can offer protection against LPS-induced acute lung injury in rats by inhibiting the expression of NF-kappaB and attenuating the production of the inflammatory cytokines.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Pharmacology
/
Bronchoalveolar Lavage Fluid
/
Lipopolysaccharides
/
Interleukin-8
/
NF-kappa B
/
Tumor Necrosis Factor-alpha
/
Rats, Wistar
/
Drug Therapy
/
Nitric Oxide Synthase Type II
Limits:
Animals
Language:
Chinese
Journal:
Journal of Southern Medical University
Year:
2007
Type:
Article
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