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Effect of Coriolus versicolor polysaccharide B on membrane glycosaminoglycans and cellular glutathione changes in RAW264.7 macrophages exposed to angiotensin II / 南方医科大学学报
Journal of Southern Medical University ; (12): 1824-1826, 2007.
Article in Chinese | WPRIM | ID: wpr-281531
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of Coriolus versicolor polysaccharide B (CVP-B) on increased membrane glycosaminoglycans (GAG) expression and intracellular glutathione (GSH) of RAW264.7 macrophages exposed to angiotensin II (Ang II).</p><p><b>METHODS</b>The plasma membrane of RAW264.7 macrophages exposed to Ang II treatment was isolated by ultracentrifugation, and the membrane GAG expression was analyzed using 1, 9-dimethylmethylene blue (DMMB) spectrophotometric assay for sulfated GAG. The intracellular reduced GSH was determined using fluorophotometry.</p><p><b>RESULTS</b>The GAG content in the macrophage membranes increased by up to 54% following cell exposure to 1.0 micromol/L Ang II, whereas in presence of 1.0 micromol;/L Ang II, CVP-B at 1, 10, and 50 microg/ml decreased the GAG content by 13%, 43% (P<0.01), and 52% (P<0.01), respectively. The macrophage GSH activity decreased by 69% following incubation with 1.0 micromol;/L Ang II for 24 h, and CVP-B treatment at 1, 10, and 50 microg/ml in presence of 1.0 micromol;/L Ang II resulted in significant increment of GSH activity by 31%(P<0.05), 104% (P<0.01), and 168% (P<0.01), respectively.</p><p><b>CONCLUSION</b>These data provide the first evidence that CVP-B inhibits elevated GAG expression in RAW264.7 macrophage membrane induced by Ang II.</p>
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Pharmacology / Polysaccharides / Angiotensin II / Cell Line / Cell Membrane / Chemistry / Agaricales / Glutathione / Glycosaminoglycans / Macrophages Limits: Animals Language: Chinese Journal: Journal of Southern Medical University Year: 2007 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Pharmacology / Polysaccharides / Angiotensin II / Cell Line / Cell Membrane / Chemistry / Agaricales / Glutathione / Glycosaminoglycans / Macrophages Limits: Animals Language: Chinese Journal: Journal of Southern Medical University Year: 2007 Type: Article