Protective effect of ischemic preconditioning against cold ischemia and reperfusion injury of rat small intestinal graft / 南方医科大学学报
Journal of Southern Medical University
;
(12): 1764-1766, 2007.
Article
in Chinese
| WPRIM
| ID: wpr-281542
ABSTRACT
<p><b>OBJECTIVE</b>To evaluate the protective effect of ischemic preconditioning against cold ischemia and reperfusion injury of rat intestinal graft following orthotopic transplantation.</p><p><b>METHODS</b>Eighty SD rats were randomly assigned into two groups with and without ischemic preconditioning, and each group was divided into 4 subgroups (n=10) according to the intestinal graft cold ischemia time of 3, 6, 12, and 18 h, respectively. Ischemic preconditioning model was established, and the small intestinal graft was preserved at 4 degrees celsius; in Ringer lactate solution for the corresponding time, followed by orthotopic transplantation of the graft. The graft samples were collected for histological examination 1 h after reperfusion, and nuclear factor-kappaB (NF-kappaB) expression in the epithelial cells was detected.</p><p><b>RESULTS</b>Ischemia preconditioning obviously relieved the histological ischemia/reperfusion injury, as shown by regular alignment of the small intestinal villi, alleviated muscular layer edema and decreased expression of NF-kappaB in the epithelia of the graft in groups with cold preservation.</p><p><b>CONCLUSION</b>Ischemic preconditioning can protect the intestinal graft from cold ischemia/reperfusion injury, and NF-kappaB is an important cytokine in ischemia preconditioning.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Organ Preservation
/
Pathology
/
Transplantation
/
Reperfusion Injury
/
NF-kappa B
/
Rats, Sprague-Dawley
/
Ischemic Preconditioning
/
Cold Ischemia
/
Intestine, Small
/
Metabolism
Type of study:
Prognostic study
Limits:
Animals
Language:
Chinese
Journal:
Journal of Southern Medical University
Year:
2007
Type:
Article
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