Hepatitis B virus P22e protein inhibits human hepatocellular carcinoma HepG2 cell apoptosis in vitro / 南方医科大学学报
Journal of Southern Medical University
;
(12): 1649-1652, 2007.
Article
in Chinese
| WPRIM
| ID: wpr-281571
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effects of the hepatitis B virus (HBV) P22e protein on the apoptosis of human hepatocellular carcinoma HepG2 cells.</p><p><b>METHODS</b>HepG2 cells were transfected with recombinant plasmid pEGFP-HBVP22e and exposed to Act-D and tumor necrosis factor alpha (TNFalpha) treatment to induce cell apoptosis. Flow cytometry was performed to determine the proportion of cells containing sub-G1 DNA to represent the number of apoptotic cells. Laser scanning confocal microscopy was used to observe the nuclear alterations in the apoptotic cells.</p><p><b>RESULTS</b>HepG2EGFP-C2HBVP22e cell strain showed a much delayed apoptosis as well as obviously lowered apoptotic rate in comparison with the HepG2 strain (P<0.01).</p><p><b>CONCLUSION</b>The introduction and expression of extraneous gene HBVP22e significantly inhibits the apoptosis of HepG2 cells.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Transfection
/
Viral Core Proteins
/
Apoptosis
/
Carcinoma, Hepatocellular
/
Hep G2 Cells
/
Hepatitis B Core Antigens
/
Metabolism
Limits:
Humans
Language:
Chinese
Journal:
Journal of Southern Medical University
Year:
2007
Type:
Article
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