Construction of a red fluorescent shuttle vector controlled by recA operon promoter of Streptococcus mutans / 中华口腔医学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To construct a red fluorescent shuttle vector controlled by recA operon promoter to transform Streptococcus mutans.</p><p><b>METHODS</b>The promoter of recA was amplified from Streptococcus mutans UA159, and connected to plasmid pDsRed2-N1 to construct pRred with a red fluorescent coding gene, which was then inserted into the shuttle vector pDL276 to construct pLRred.</p><p><b>RESULTS</b>pLRred was successfully constructed, and Escherichia coli transformed with the pLRred plasmid could express reporter gene DsRed.</p><p><b>CONCLUSIONS</b>The recombination plasmid pLRred can be used in the further research of the expression of cariogenic virulence factor gene by Streptococcus mutans in biofilm.</p>