Authentification of Radix Cyathule and its adulterants based on SCAR markers / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 953-956, 2010.
Article
in Chinese
| WPRIM
| ID: wpr-281680
ABSTRACT
Chuanniuxi (Radix Cyathule) is one of the most important geo-herb in Sichuan province, which adulterants are Hongniuxi (Cyathula capitata) and Huainiuxi (Achyranthes bidentata). In this paper Chuanniuxi and its adulterants were identified by SCAR markers. Nineteen populations from Tianquan, Baoxin, Huili and Jinkouhe were collected and their RAPD fingerprints were established. Based on the RAPD patterns, two polymorphic bands F300 and F500 were selected, recycled, cloned and sequenced. According to the sequences two pairs of sequence characterized amplified regions (SCAR) primers were designed and used to amplify all materials to prove the efficiency of identification of the different populations. Chuanniuxi and Huiniuxi could be distinguished by the primer SC-320, Chuanniuxi and Hongniuxi could be distinguished by the primer SC-495. Combining the two SCAR markers, Chuanniuxi, Hongniuxi and huainiuxi could be identified effectively and quickly.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Polymorphism, Genetic
/
Time Factors
/
Genetic Markers
/
Drug Contamination
/
Cloning, Molecular
/
Sequence Analysis, DNA
/
DNA, Plant
/
Random Amplified Polymorphic DNA Technique
/
Nucleic Acid Amplification Techniques
/
Amaranthaceae
Type of study:
Prognostic study
Language:
Chinese
Journal:
China Journal of Chinese Materia Medica
Year:
2010
Type:
Article
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