Effect of Dureping injection on TIR signal pathway on Ana-1 cells / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the influence of Dureping injection to the murinal celiac macrophage Ana-1 on TIR signal pathway.</p><p><b>METHOD</b>Ana-1 cell line was infected by influenza virus FM1 strain and treated with the Dureping injection in different concentrations (10.1 mg x L(-1) group) for 12 h and 24 h. Then we collected the cells, extracted mRNA and measured the expressions of TLR7, MyD88, IRAK4, TRAF6 and NF-kappaB p65 respectively by RT-PCR.</p><p><b>RESULT</b>Dureping injection down-regulated the expression of TLR7, MyD88, IRAK4, TRAF6 and NF-kappaB p65 mRNA in Ana-1 cell line infected by influenza virus, in a dose-dependent manner significantly.</p><p><b>CONCLUSION</b>Dureping injection has an obvious effect against influenza virus FM1 strain by regulating the TIR signal pathway.</p>