Establishing DNA chip technique for detecting hepatitis C virus genotypes and primary application / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
;
(6): 375-377, 2003.
Article
in Chinese
| WPRIM
| ID: wpr-281778
ABSTRACT
<p><b>OBJECTIVE</b>To establish a new method based DNA chip technique for detecting HCV genotypes.</p><p><b>METHODS</b>Genotyping probes were designed according to the sequence of HCV 5' NCR to generate DNA chip. The probes on DNA chip contains 5 major genotypes and 8 subtypes. The DNA fragment amplified by labeling Cy5 fluorescence was hybridized with DNA chip.</p><p><b>RESULTS</b>Fifty-five out of 65 isolates detected by DNA chip belonged to 1b- DNA sequencing of form a part of the isolates was used as the control. The results of both were completely consistent.</p><p><b>CONCLUSION</b>The method is simple and rapid with high specificity and sensitivity. It can be applied in detection of HCV RNA and genotypes.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Blood
/
RNA, Viral
/
Polymerase Chain Reaction
/
Sensitivity and Specificity
/
Classification
/
Hepacivirus
/
5' Untranslated Regions
/
Oligonucleotide Array Sequence Analysis
/
Genetics
/
Genotype
Type of study:
Diagnostic study
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Experimental and Clinical Virology
Year:
2003
Type:
Article
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