Construction of a tRNAVal promoter plasmid expressing shRNAs in mammalian cells which mediates RNA interference / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
;
(6): 358-360, 2003.
Article
in Chinese
| WPRIM
| ID: wpr-281783
ABSTRACT
<p><b>OBJECTIVE</b>Constructing a plasmid containing tRNAVal promoter to express shRNA which mediates RNA interference.</p><p><b>METHODS</b>A tRNAVal gene was amplified from human genomic DNA by PCR and replaced the last several bases of 3' end by a linker. The tRNAVal promoter after artificial mutation followed a shRNA sequence to luciferase was cloned into pUC18, Puc-tRNAVal, lucRi Cotransfected with pMAMneoLuc into BHK-21 cell to detect the effect of luciferase expression.</p><p><b>RESULTS</b>pUC-tRNAVallucRi suppressed the luciferase expression from pMAMneoLuc by 97.9%-9.5%.</p><p><b>CONCLUSION</b>The results showed that the tRNAVal shRNA plasmid could efficiently suppress luciferase expression in BHK-21.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Plasmids
/
RNA, Transfer
/
Cell Line
/
Promoter Regions, Genetic
/
RNA Interference
/
Genetic Vectors
/
Genetics
/
Luciferases
Limits:
Animals
/
Humans
Language:
Chinese
Journal:
Chinese Journal of Experimental and Clinical Virology
Year:
2003
Type:
Article
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