Nucleotides sequence analysis of EV71 Chinese strain SHZH98 3C segment / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
;
(6): 354-357, 2003.
Article
in Chinese
| WPRIM
| ID: wpr-281784
ABSTRACT
<p><b>OBJECTIVE</b>To study the EV71 Chinese strain SHZH98 and analyze its genetic evolution using 3c gene as index.</p><p><b>METHODS</b>The 3C gene cDNA of EV71 Chinese strain SHZH98 was amplified by PCR, the PCR product was sequenced.</p><p><b>RESULTS</b>The EV71 Chinese mainland strain SHZH98 3C segment was 549 bps in length. Comparison of nucleotide sequences from other enteroviruses which have been published, revealed a higher homology to strain MS, 78.7% at nucleotide level and 93.45% at deduced amino acid level. The homology to strain BrCr was 76.7% at nucleotide level and 89.1% at deduced amino acid level. Taiwan strains POLY,NCKU,TW2086,TW2272 shared a lower homology with Chinese mainland strain SHZH98, 74.0%, 73.8%, 71.9%, 69.8% at nucleotide level and 90.7%, 90.2%, 84.2%, 82.5% at deduced amino acid level. The genetic progress analysis revealed that EV71 Chinese mainland strain SHZH98 3C segment shares more homology with European and American strains than Taiwan strains.</p><p><b>CONCLUSION</b>The non-structural protein of EV71 Chinese strains may have different evolutionary process from Taiwan strains.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Molecular Sequence Data
/
Base Sequence
/
Sequence Homology, Nucleic Acid
/
Amino Acid Sequence
/
Sequence Homology, Amino Acid
/
Enterovirus
/
Reverse Transcriptase Polymerase Chain Reaction
/
Genes, Viral
/
Genetics
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Experimental and Clinical Virology
Year:
2003
Type:
Article
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