Construction and immune potency of recombinant adenovirus containing codon-modified HIV-1 gp120 / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
;
(6): 113-117, 2004.
Article
in Chinese
| WPRIM
| ID: wpr-281840
ABSTRACT
<p><b>BACKGROUND</b>To construct replication-deficient recombinant adenovirus expressing wild and codon-modified HIV-1 gp120.</p><p><b>METHODS</b>The viral codons were changed to the codon usage of highly expressed mammal gene, the resulting modified gp120 gene was synthesized. The wild and modified gp120 genes were cloned into shuttle vector pShuttle-CMV respectively, and then the constructed plasmids containing gp120 gene was cotransformed with the backbone vector pADeasy-1 into E.coli BJ5183. Transfection of the recombinant AdEasy plasmid into 293 cells was performed to obtain recombinant adenoviruses. The mice were immunized with the recombinant adenoviruses. Their immunogenicity was evaluated by testing antibody and CTL levels of immunized mice.</p><p><b>RESULTS</b>Two strains of recombinant adenovirus expressing wild and codon-modified HIV-1 gp120 were obtained. The protein expressing level of the recombinant adenoviruses containing modified genes was much higher than that containing wild genes. The mice immunized with recombinant adenoviruses elicited HIV-1 specific antibody and CTL response. The rAd-mod gp120 group was better than the rAd-wt gp120 group.</p><p><b>CONCLUSION</b>Replication-deficient recombinant adenovirus expressing HIV-1 gp120 can elicit HIV-1 specific humoral and cellular response, the codon-modified recombinant virus was more efficient than the native.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Plasmids
/
Recombination, Genetic
/
Blood
/
Codon
/
T-Lymphocytes, Cytotoxic
/
HIV Antibodies
/
HIV Envelope Protein gp120
/
Adenoviridae
/
HIV-1
/
AIDS Vaccines
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Experimental and Clinical Virology
Year:
2004
Type:
Article
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