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Synergetic transactivating effect of HCV core and HBV X proteins on SV40 early promoter/enhancer / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology ; (6): 70-72, 2003.
Article in Chinese | WPRIM | ID: wpr-281851
ABSTRACT
<p><b>BACKGROUND</b>To investigate the synergetic transactivating effects of HCV core and HBV X proteins.</p><p><b>METHODS</b>HCV core and HBV X protein-expressing plasmids were constructed with the vector pcDNA3.1(-). The plasmids were transfected into HepG2 cells and cotransfected Hep2 cells with reporter plasmid Psv-lacZ by lipofectamine plus reagents. The virus proteins produced in transient expression system were detected at the transcription and translation levels. The activity of b-galactosidase was detected, which reflected the transactivating function of the proteins.</p><p><b>RESULTS</b>The expression of plasmids were detected in soluble protein cell extracts of transiently transfected HepG2 cells. HCV core protein activated the b-galactosidase expression at a value 4.9 times higher than the control, while HBV X protein activated at a value 3.5 times. It arrived at 9 times transfected with the plasmids simultaneously. The activating effect increased in relation to the amount of plasmids.</p><p><b>CONCLUSIONS</b>The results suggested that the two kinds of virus proteins have transactivating effect on SV40 early promoter/enhancer, and they acted synergistically. These contribute to explain the mechanisms of liver injury or tumorigenesis induced by HCV or/and HBV infection.</p>
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Virology / Transcriptional Activation / Trans-Activators / Viral Core Proteins / Enhancer Elements, Genetic / Promoter Regions, Genetic / Beta-Galactosidase / Hepacivirus / Carcinoma, Hepatocellular / Simian virus 40 Limits: Animals / Humans Language: Chinese Journal: Chinese Journal of Experimental and Clinical Virology Year: 2003 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Virology / Transcriptional Activation / Trans-Activators / Viral Core Proteins / Enhancer Elements, Genetic / Promoter Regions, Genetic / Beta-Galactosidase / Hepacivirus / Carcinoma, Hepatocellular / Simian virus 40 Limits: Animals / Humans Language: Chinese Journal: Chinese Journal of Experimental and Clinical Virology Year: 2003 Type: Article