Activating effect of hepatitis B virus preS/S protein on proliferating cell nuclear antigen gene promoter / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
;
(6): 42-45, 2003.
Article
in Chinese
| WPRIM
| ID: wpr-281858
ABSTRACT
<p><b>BACKGROUND</b>PreS/S gene was derived from hepatitis B virus (HBV) integration fragment of human hepatocellular carcinoma genome, containing the promoter of preS/S and the C terminal truncated preS/S open reading frame. PreS/S protein may have important roles in processing hepatoma in some HBV-infected patients. The aim of the study was to study the activity of HBV preS/S protein on proliferating cell nuclear antigen (PCNA) promoter and to localize compartment of the preS/S protein in the liver cell line L02.</p><p><b>METHODS</b>The authors studied the effect of the 3 -truncated preS/S on human PCNA promoter by co-transfecting the expression plasmids of luciferase reporter gene, used the immunohistochemical method to localize the preS/S protein.</p><p><b>RESULTS</b>The expression product of the plasmid, pKSH7C-HpaI which contained the 3 -truncated preS/S and the flanking cellular sequences, stimulated the expression of PCNA promoter dose dependently,and its effect was 0.5 folds higher than control. Immunohistochemistry showed that the preS/S protein located in the cytosolic region of the liver cell line L02.</p><p><b>CONCLUSIONS</b>The HBV preS/S protein could stimulate the PCNA promoter of the liver cell, its effect was not direct, which suggests that the effect of preS/S protein on PCNA promoter was probably through the cell signal transduction pathway.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Protein Precursors
/
Virology
/
Tumor Cells, Cultured
/
Hepatitis B virus
/
Promoter Regions, Genetic
/
Virus Integration
/
Carcinoma, Hepatocellular
/
Proliferating Cell Nuclear Antigen
/
Genetics
/
Hepatitis B Surface Antigens
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Experimental and Clinical Virology
Year:
2003
Type:
Article
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