Effects of decitabine against acute T lymphoblastic leukemia cell line Molt4 / 中华血液学杂志
Chinese Journal of Hematology
;
(12): 230-234, 2015.
Article
in Chinese
| WPRIM
| ID: wpr-282065
ABSTRACT
<p><b>OBJECTIVE</b>To explore the effects and possible mechanisms of decitabine on Molt4 in vitro.</p><p><b>METHODS</b>Effects of decitabine on cells proliferation were detected by using CCK-8, the apoptosis by Annexin V-FITC, cell cycles by propidium iodide-FACS. Discrepancy genes were screened by RNA-seq technique. The CpG methylation of lactoferrin (LTF) gene in Molt4 cells were identified by Bisulfite sequencing PCR (BSP). The expression of LTF mRNA in Molt4 by RT-PCR and LTF protein expression were analyzed by Western blot.</p><p><b>RESULTS</b>Decitabine effectively inhibited proliferation and induced apoptosis for Molt4 cells by an time- and dose-dependent manners. Cell cycles were arrested at the G₀/G₁ phase. The promoter methylation degree of LTF gene in Molt4 cells was 72.3% before decitabine treatment and decreased to 45.0% after treatment with 0.50 μmol/L decitabine for 72 h. After the reduction of methylation, expression of its mRNA and protein increased, meanwhile caspase 3 and caspase 9 protein expression levels increased.</p><p><b>CONCLUSION</b>The demethylating drug decitabine can induce apoptosis, detain cell cycle at phase G₀/G₁, inhibit proliferation and up-regulate LTF gene expression in Molt4 cells. LTF may become a new target for acute T lymphoblastic leukemia.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Azacitidine
/
Cell Cycle
/
Promoter Regions, Genetic
/
Apoptosis
/
DNA Methylation
/
Cell Line, Tumor
/
Cell Proliferation
/
Caspase 3
/
Precursor T-Cell Lymphoblastic Leukemia-Lymphoma
/
Lactoferrin
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Hematology
Year:
2015
Type:
Article
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