pcDNAL1 genetic immunization can induce specific cell-mediated immune responses in C57BL/6 mice / 中华医学杂志(英文版)
Chinese Medical Journal
;
(24): 1697-1700, 2002.
Article
in English
| WPRIM
| ID: wpr-282106
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the specific cell-mediated immune efficacy of the an HPV16 prophylactic vaccine.</p><p><b>METHODS</b>C57BL/6 mice were randomly divided into 3 groups experimental group I (treated with pcDNA L1), control group II (treated with pcDNA3.1) and control group III (treated with PBS buffer). The mice were immunized three times during a three-week interval. Ten to fourteen days after the third inoculation, a footpad swelling test was used to detect delayed-type hypersensitivity (DTH) responses. Antigen-specific splenocyte proliferation assay and quantitation of IFN-gamma cells in splenocytes were performed by FACS assay.</p><p><b>RESULTS</b>In the experimental group, splenocytes actively proliferated after stimulation with HPV16 VLP, and had developed a markedly larger amount of CD8(+) IFN-gamma(+) cells, which is an index for special CTL. Also, the footpad was significantly thickened upon inoculation with HPV16 VLP.</p><p><b>CONCLUSION</b>Naked DNA vaccine of HPV16 L1 can induce specific cell-mediated immune responses in mice, which should be considered for evaluation of HPV16 DNA vaccine feasibility.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Papillomaviridae
/
Spleen
/
Viral Vaccines
/
Lymphocyte Activation
/
Immunization
/
Interferon-gamma
/
Vaccines, DNA
/
Cell Biology
/
Allergy and Immunology
/
Hypersensitivity, Delayed
Limits:
Animals
Language:
English
Journal:
Chinese Medical Journal
Year:
2002
Type:
Article
Similar
MEDLINE
...
LILACS
LIS