The cross-reactivity of I-A(g7) and I-A(d) tetramers / 中华医学杂志(英文版)
Chinese Medical Journal
;
(24): 1579-1581, 2002.
Article
in English
| WPRIM
| ID: wpr-282135
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the cross-reactivity between glutamic acid decarboxylase (GAD)-I-A(g7) and I-A(d) tetramer in diabetes-prone non-obese diabetic (NOD) mice (I-A(g7)) and diabetes-free Balb/c mice (I-A(d)).</p><p><b>METHODS</b>Two GAD peptide I-A(g7) and I-A(d) tetramers were generated and compared for phenotype and function of sorted GAD peptide I-A(g7) and I-A(d) tetramer-positive (tet+) T cells.</p><p><b>RESULTS</b>The cross-reactivity is shown in either tetramer positive percentage or tetramer staining intensity. The NOD and Balb/c derived-tet+ T cells were able to be cross-stained by GAD peptide I-A(g7) and I-A(d) tetramers, and responded to both irradiated NOD and Balb/c splenotyes under stimulation by synthetic and recombinant GAD peptides.</p><p><b>CONCLUSION</b>Although I-A(g7) and I-A(d) are closely related in biochemical and biological aspects, their most notable difference is the presence or absence of a negatively charged residue at position beta57 that links to insulin-dependent diabetes mellitus.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Molecular Sequence Data
/
T-Lymphocytes
/
Histocompatibility Antigens Class II
/
Amino Acid Sequence
/
Mice, Inbred NOD
/
Cross Reactions
/
Allergy and Immunology
/
Glutamate Decarboxylase
/
Mice, Inbred BALB C
Limits:
Animals
Language:
English
Journal:
Chinese Medical Journal
Year:
2002
Type:
Article
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