Study of isolation of fluoroquinolone-resistant Ureaplasma urealyticum and identification of mutant sites / 中华医学杂志(英文版)
Chinese Medical Journal
;
(24): 1573-1575, 2002.
Article
in English
| WPRIM
| ID: wpr-282137
ABSTRACT
<p><b>OBJECTIVE</b>To study the resistance mechanism of clinical isolates of Ureaplasma urealyticum resistant to fluoroquinolones.</p><p><b>METHODS</b>Thirteen isolates of Ureaplasma urealyticum resistant to six fluoroquinolones were selected out of 184 clinical isolates and their QRDRs (quinolone resistance-determining region) gyrA, gyrB, parC and parE were amplified by PCR. Sequencing results were compared to those susceptible reference strains and a comparison of deduced amino acid sequences were performed.</p><p><b>RESULTS</b>Sequence comparison revealed a C to A change at 87nt of gyrA QRDR leading to the substitution of Asp95 with glutamic acid and a C to T change at 50nt of parC QRDR leading to the substitution of Ser80 with leucine.</p><p><b>CONCLUSION</b>These results suggest that a C to A change at 87nt of gyrA QRDR and a C to T change at 50nt of parC QRDR are associated with fluoroquinolone resistance of Ureaplasma urealyticum.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Polymerase Chain Reaction
/
Ureaplasma urealyticum
/
Amino Acid Substitution
/
Fluoroquinolones
/
DNA Gyrase
/
DNA Topoisomerase IV
/
Drug Resistance, Multiple, Bacterial
/
Genetics
/
Anti-Infective Agents
Type of study:
Diagnostic study
Limits:
Humans
Language:
English
Journal:
Chinese Medical Journal
Year:
2002
Type:
Article
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