Alpha-synuclein immunoreactivity and ultrastructural study of glial cytoplasmic inclusions in multiple system atrophy / 中华医学杂志(英文版)
Chinese Medical Journal
;
(24): 1491-1495, 2002.
Article
in English
| WPRIM
| ID: wpr-282157
ABSTRACT
<p><b>OBJECTIVE</b>To understand the possible pathogenesis of sporadic multiple system atrophy (MSA).</p><p><b>METHODS</b>The immunoreactivity and ultrastructural features of glial cytoplasmic inclusions (GCIs) in 12 autopsy patients with MSA and 4 normal control groups were studied. All regional sections from each subject were evaluated with HE staining, Klüver-Barrera (KB), Holzer's, modified Gallyas-Braak's (GB) methods and immunohistochemical staining with alpha-synuclein and ubiquitin antibodies. Pontine white matter with abundant GCIs from case 1 was examined, using conventional electron microscopy, Gallyas-Braak's electron microscopy and immunoelectron microscopy.</p><p><b>RESULTS</b>The presence of GCIs as constantly demonstrated in all MSA patients. Strong alpha-synuclein immunoreactivity was observed in all of the ubiquitinated GCIs. However, the density of alpha-synuclein positive GCIs differed from case to case, and there was no relationship between the density of GCIs and age, sex, or MSA subtype. Ultrastructural features indicated that argyrophilic granule-associated filaments of about 25 nm in diameter were the predominant constituents of GCIs, and the anti alpha-synuclein antibody selectively labeled in these filaments. No GCIs and alpha-synuclein immunoreaction were found in control brain tissues.</p><p><b>CONCLUSIONS</b>GCI was a pathognomonic change in sporadic MSA patients. Accumulation of alpha-synuclein in GCIs may occur during the early stags of MSA. Seletcive alpha-synuclein positive abnormal microtubules in GCIs therefore play an important role in the pathogenesis of MSA.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Immunohistochemistry
/
Inclusion Bodies
/
Neuroglia
/
Microscopy, Immunoelectron
/
Multiple System Atrophy
/
Synucleins
/
Alpha-Synuclein
/
Metabolism
/
Nerve Tissue Proteins
Limits:
Aged
/
Aged80
/
Female
/
Humans
/
Male
Language:
English
Journal:
Chinese Medical Journal
Year:
2002
Type:
Article
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