Analysis of population genetic structure and molecular identification of Changium smyrnioides and Chuanminshen violaceum with ISSR marker / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 598-603, 2003.
Article
in Chinese
| WPRIM
| ID: wpr-282217
ABSTRACT
<p><b>OBJECTIVE</b>To assess the population genetic diversity and genetic structure and screen species-specific bands for identification of Changium smyrnioides and Chuanminshen violaceum.</p><p><b>METHOD</b>Seven wild populations of Changium smyrnioides and one cultivated population of Chuanminshen violaceum were studied by ISSR analysis. The population genetic diversity and population genetic structure were assessed by using POPGENE software.</p><p><b>RESULT</b>A total of 152 ISSR markers were scored, among which 136 (90.8%) were polymorphic. The values of Gst tended to be high (mean Gst = 0.575). The level of genetic divesity of Changium smyrnioides (A = 1.272; P = 27.26%; I = 0.132; H = 0.087) was higher than that of Chuanminshen violaceum (A = 1.217; P = 21.7; I = 0.103; H = 0.067).</p><p><b>CONCLUSION</b>The genetic variation of Changium smyrnioides is high and the majority of genetic variation occur among populations. Substantial genetic divergence is shown by cluster analysis (UPGMA) to befound between Changium smyrnioides and Chuanminshen violaceum at DNA level. In addition, one species-specific marker has been obtained in Chuanminshen violaceum. The phylogenetic relationship of two species has also been discussed.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Phylogeny
/
Plants, Medicinal
/
Polymorphism, Genetic
/
Species Specificity
/
Repetitive Sequences, Nucleic Acid
/
Genetic Markers
/
Cluster Analysis
/
China
/
Polymerase Chain Reaction
/
Classification
Type of study:
Diagnostic study
/
Prognostic study
Country/Region as subject:
Asia
Language:
Chinese
Journal:
China Journal of Chinese Materia Medica
Year:
2003
Type:
Article
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