Effects of selenium and B-27 supplements on viability and differentiation of neural stem cell in newborn rat / 中华预防医学杂志
Chinese Journal of Preventive Medicine
;
(12): 324-327, 2005.
Article
in Chinese
| WPRIM
| ID: wpr-282338
ABSTRACT
<p><b>OBJECTIVE</b>To assess how trace element selenium and B27 supplements affect the neural stem cell (NSc) differentiation in vitro.</p><p><b>METHODS</b>The development and differentiation of NSc from the newborn rat were observed with primary culture and subculture during treating by sodium-selenite, and selenium-methyl-cysteine (SMC). The immunocytochemistry techniques were used to identify the NSc and mature protein expression with neuron marker beta-tubulin, astrocyte marker GFAP, and oligodendrocyte marker CNPase. The neurosphere morphology and neurite outgrowth were observed.</p><p><b>RESULTS</b>Adding the complete B-27 serum-free supplement, Selenium could promote the neurosphere viability, development and differentiation. Without selenium and B-27, neurosphere could not survive and differentiate. Without B-27 in the medium but there containing selenium, the neurosphere could promote the viability and development into neuron, astrocyte and oligodendrocyte, as compared with the no-containing B-27 and selenium groups, these differentiated cells might have more quantity, more branches and better morphological nerve net. The count of the neuron, astrocyte and oligodendrocyte was 11.2/Hp, 16.1/Hp and 9.3/Hp.</p><p><b>CONCLUSIONS</b>The selenium should be very important for neural stem cells' survival. Selenium could promote the neurosphere cells differentiation and development.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Selenium
/
Stem Cells
/
Tubulin
/
Immunohistochemistry
/
Cell Differentiation
/
Cell Survival
/
Cells, Cultured
/
Organoselenium Compounds
/
Culture Media, Serum-Free
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Preventive Medicine
Year:
2005
Type:
Article
Similar
MEDLINE
...
LILACS
LIS