Effects of phytoestrogens on gap junctional intercellular communication / 中华预防医学杂志
Chinese Journal of Preventive Medicine
;
(12): 126-128, 2005.
Article
in Chinese
| WPRIM
| ID: wpr-282374
ABSTRACT
<p><b>OBJECTIVE</b>To observe effects of phytoestrogens quercetin (QC), Genistein (GEN), coumestrol (COM), and enterolactone (ENL) on gap junctional intercellular communication (GJIC) in HaCaT cells.</p><p><b>METHODS</b>HaCaT cells were exposed to QC, GEN, COM, and ENL at 0.1, 1.0, 10.0 and 100.0 micromol/L for 24 hours. The effects of phytoestrogens on GJIC were determined by fluorescence redistribution after photobleaching (FRAP) technique of using a laser scanning confocal microscope (LSCM).</p><p><b>RESULTS</b>QC did not affect the GJIC at 0.1-10.0 micromol/L, whereas, GEN, COM, and ENL exhibited inhibition on the GJIC in some extent at 0.1-10.0 micromol/L without showing significant cytotoxicity. The ratio of fluorescence recovery were between 31.77% to 37.06%, which were significantly decreased compared the vehicle control (44.74%).</p><p><b>CONCLUSION</b>The phytoestrogens GEN, COM, and ENL, but not QC, could inhibit the GJIC function in HaCaT cells at concentrations could be reached in human serum in some instance, indicating they could, under certain conditions, be cancer promoters. Therefore, it should be prudent to use these chemicals as pharmaceuticals or dietary supplements.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Physiology
/
Quercetin
/
Cell Communication
/
Cell Line
/
Gap Junctions
/
Microscopy, Confocal
/
Genistein
/
Coumestrol
/
Phytoestrogens
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Preventive Medicine
Year:
2005
Type:
Article
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