Pharmacological effects of serum containing chinese medicine Bushen Huayu Jiedu Compound Recipe in lung cancer drug-resistance cells / 中国结合医学杂志
Chinese journal of integrative medicine
;
(12): 46-50, 2008.
Article
in English
| WPRIM
| ID: wpr-282381
ABSTRACT
<p><b>OBJECTIVE</b>To explore the pharmacologic effects of Chinese medicine Bushen Huayu Jiedu Compound Recipe (BSHYJDR) in drug-resistance cells of lung cancer.</p><p><b>METHODS</b>Human lung adenocarcinoma A549/DDP cell strain was selected, serum pharmacology and flow cytometer (FCM) method were adopted, S180 tumor-bearing mice and normal mice were given, through gastrogavage, different doses of a decocted concentration of BSHYJDR. Serum from the abdominal aorta was taken to observe the effect of drug-serum on cisplatin (DDP) concentration, free Ca2+ concentration and the expression of lung drug-resistance protein LRP-56 in A549/DDP cells.</p><p><b>RESULTS</b>Compared with the drug-resistance group, the intracellular DDP concentration in the group taking a high dose and the normal group of Chinese medicine showed significant difference (P<0.05), while no significant difference was found in the low-dose group (P>0.05). Compared with the drug-resistance group, the Ca2+ concentration in cells and the expression of LRP in lung cancer drug-resistance cells A549/DDP of the high-dose group, the low-dose group and the normal group of Chinese medicine were significantly different (all P<0.01), the LRP expression of the normal group was obviously higher than that of the drug-resistance group (P<0.05).</p><p><b>CONCLUSION</b>It was indicated that serum containing Chinese medicine BSHYJDR in the tumor-bearing mice and the normal mice had certainly different, tumor-bearing mice serum containing could improve drug concentration in lung cancer drug-resistance cells, prevent the inflow and release of Ca2+, and inhibit the expression of the drug-resistance gene in the lung cancer drug-resistance cells, which might be the mechanism of BSHYJDR in enhancing the efficacy in reversing and inhibiting tumor.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Pharmacology
/
Calcium
/
Cisplatin
/
Drug Resistance, Neoplasm
/
Vault Ribonucleoprotein Particles
/
Cell Line, Tumor
/
Drug Therapy
/
Genetics
/
Lung Neoplasms
Type of study:
Diagnostic study
Limits:
Animals
/
Humans
Language:
English
Journal:
Chinese journal of integrative medicine
Year:
2008
Type:
Article
Similar
MEDLINE
...
LILACS
LIS